Fig. 5.

Characterization of four AAVs obtained from the U Penn viral vector core for their ability to transduce Area X neurons. In all panels, green signals show GFP, red signals show NeuN, and blue signals show DAPI staining. a Left photomicrograph reveals that AAV2/5 transfects large portions of the brain except for Area X. Scale bar 1000 μm. Right higher magnification shows robust transduction in the striatopallidum outlying Area X. Scale bar 50 μm. b AAV2/1 shows robust transduction but also induces lesions at the injection site. Scale bar 500 μm. c Representative photomicrograph shows that AAV2/rh10 has a low transfection rate in Area X and induces a lesion at the injection site. Scale bar 500 μm. d Right photomicrograph shows low levels of transduction by AAV2/8. Scale bar 500 μm. Even at higher magnification (right), there were very few GFP-positive cell bodies in the most densely transfected region. Scale bar 100 μm