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. Author manuscript; available in PMC: 2017 Oct 3.
Published in final edited form as: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2017 Mar 7;203(9):691–706. doi: 10.1007/s00359-017-1153-0

Fig. 6.

Fig. 6

U Penn AAV2/1-CB7-GFP retrogradely infects at least one, if not more, Area X afferent nuclei. a High numbers of GFP-positive cells are visible at the injection site in Area X (dotted outline). Scale bar 500 μm. b High numbers of YFP-positive cells (effectively labeled by an anti-GFP antibody) were also identified in LMAN (dot- ted outline). Scale bar 500 μm. c The near perfect fill of HVC is best explained by retrograde transfection from Area X. Scale bar 500 μm. d Retrograde transfection was further confirmed by expression of GFP-positive fibers in RA, which does not receive input from Area X but does receive input from LMAN and HVC. Scale bar 200 μm