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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Biol Methods Protoc. 2017 Jan 25;2(1):bpw006. doi: 10.1093/biomethods/bpw006

Primers Sense Antisense

Spike 5′-GCTATCACAGTCCTGCGCTG-3′ 5′-CGGGCAGTAAACCTTCTCATGA-3′
ASF1 5′-CTTTCGTGTGGGTCGGTAGT-3′ 5′-CCCCTAACACTTCCTGCGTA-3′
VSG 224 5′-CGATGACGTCAATCCAGATG-3′ 5′-CCGTTGGTGTCGTGTCTTC-3′
cSSR - 1 5′-GTATCACCACAGCCCGAACT-3′ 5′-GGCAACCGAAAACAAAGAAA-3′
cSSR - 2 5′-AATTCGCCTACTGTCCATGCCGAT-3′ 5′-TGTGCAGAACGCACATAAGGCAAC-3′
cSSR - 3 5′-GGTAAAGCTGGCGAAGTTGAAGGT-3′ 5′-TTTCTTCCGGACACTCGCGATCAT-3′
cSSR - 4 5′-GGCCTTTATCCGCCGAAATTGGTT-3′ 5′-CACTTTGTGGTGAATCAGCGGCAT-3′
cSSR - 5 5′-AACAACAGACTAATGGCGGG-3′ 5′-TCGATGAATCTGCGCACTAC-3′
dSSR - 1 5′-CCCAATTTCACGGAAGAAAA-3′ 5′-CTTGTGGACACGTGACTGCT-3′
dSSR - 2 5′-CGACCCAGCATAATGTTCCT-3′ 5′-GGAAAGTGGACCGTTTTGAA-3′
dSSR - 3 5′-AAGCGGCGTCATTATTTGCAGACG-3′ 5′-ATTGCTTCCACACCAACCAACGAC-3′
dSSR - 4 5′-TTCACGTGAGAGGTGCATTCCAGT-3′ 5′-ACCATGCCGAATTCAGTTGTACCG-3′
dSSR - 5 5′-CACCCAATCCGTCATTCCACATCA-3′ 5′-ACAGTCACAGCTCTCCTTCTCACA-3′
dSSR - 6 5′-TTCGTGTCAACAGGAGGTGCACTA-3′ 5′-ACAGATGCCGTAGGTTCATTCGGT-3′