Fig. 6.

Effect of treatment on glutamine transport and relevant signaling pathways and apoptosis. a HMVP2 cells were treated for 2 h with 20 µM CUR, UA, RES or their combinations of two compounds. The levels of the glutamine transporter ASCT2, and phospho and total protein levels for Src, STAT3, AMPK, p70S6K and S6 were measured by Western blotting. Western blotting was performed two times with β-actin controls for each experiment. Numbers above blots indicate band intensities (normalized to control). b The percent of apoptotic cells (measured by Annexin V) and the levels of apoptotic markers (Western blotting) were probed after 24 h of treatment with the natural compounds. One-way ANOVA with significance at p < 0.05 was used. Statistical significance is shown as different from control (a), CUR (b) and UA (c)