| Photo-Flo 200 |
Kodak |
1464510 |
|
| 16% Paraformaldehyde (formaldehyde aqueous solution) |
Electron Microscopy Sciences |
RT 15710 |
Dilute 16% solution into 4% working stocks with 1X PBS and freeze aliquots at −20 °C |
| Teflon printed 3 ring slides |
Electron Microscopy Sciences |
63418-11 |
|
| Premium uncharged frosted end glass slides |
Several commercial brands |
|
Clean slides in ethyl or isopropyl alcohol and allow to drip dry prior to use; label slides on frosted end with a permanent marker or pencil depending on subsequent use of slides |
| Surgical scissors (sharp and blunt tips) |
Fine Science Tools |
14001-12 |
Different brand of a similar type will work |
| Fine tip surgical scissors (sharp tips) |
Fine Science Tools |
14058-11 |
Different brand of a similar type will work |
| Straight fine tip forceps |
Fine Science Tools |
11050-10 |
Different brand of a similar type will work |
| Curved medium tip forceps |
Fisher |
16100110 |
Different brand of a similar type will work |
| Scalpel handle |
Fine Science Tools |
10003-12 |
Different brand of a similar type will work |
| Mouse anti-SYCP3 |
Abcam |
ab97672 |
Use at 1:300 |
| Anti-centromere protein (derived from human CREST patient serum) |
Antibodies Incorporated |
15-234-0001 |
Use at 1:50 |
| Humidified chamber |
|
|
We use Nunc square culture dishes 500 cm2/well with moistened paper towels |
| Widefield microscope with epifluorescence |
Leica microsystems |
|
Any standard model |
| Coplin jars |
Several commercial brands |
|
50 ml capacity; 40 mm (1.6 in.) diameter, holds 10 slides (back to back) |
| Petri dishes |
Several commercial brands |
|
100 × 15 mm diameter, polystyrene sterile untreated |
