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. Author manuscript; available in PMC: 2017 Nov 27.
Published in final edited form as: J Endocr Soc. 2017 Mar 8;1(4):345–358. doi: 10.1210/js.2016-1119

Table 2.

Primers Used to Generate the Human GHR Variants, c.964dupG and c.920_921ins14

Primer Name Primer Sequence
Primers used to generate the hGHR c.964dupG variant
 hGHR c.964dupG forward 5′ GGAAGGAAAATTAGAGGAGGGTGAACACAATCTTAGCCATT 3′
 hGHR c.964dupG reverse 5′ AATGGCTAAGATTGTGTTCACCCTCCTCTAATTTTCCTTCC 3′
Primers used to generate the hGHR c.920_921ins14 variant
 hGHR c.920_921ins14 (step 1) forward 5′ CCCCAGTTCCAGTTCCAAAGATTAATCTCAAGGAATCGATCCA 3′
 hGHR c.920_921ins14 (step 1) reverse 5′ TGGATCGATTCCTTGAGATTAATCTTTGGAACTGGAACTGGGG 3′
 hGHR c.920_921ins14 (step 2) forward 5′ CAGTTCCAGTTCCAAAGATTAATCTCAAAGATAGGAATCGATCCAG 3′
 hGHR c.920_921ins14 (step 2) reverse 5′ CTGGATCGATTCCTATCTTTGAGATTAATCTTTGGAACTGGAACTG 3′
 hGHR c.920_921ins14 (step 3) forward 5′CCAGTTCCAAAGATTAATCTCAAAGATTACAAGGAATCGATCCGATC 3′
 hGHR c.920_921ins14 (step 3) reverse 5′ GATCTGGATCGATTCCTTGTAATCTTTGAGATTAATCTTTGGAACTGG 3′

Nucleotides inserted are underlined.