Hpl proteins localize to macronuclear chromatin, new developing macronuclei, and pycnotic nuclei. YFP fusion proteins were expressed by induction with CdCl2 and visualized by epifluorescence microscopy during regular growth, 5 hours into conjugation, and 9 hours into conjugation. Schematic at the top shows arrangements of various nuclei. DIC images on the left of each image pair show positions of nuclei. “NS”, not shown; “NM”, new macronucleus; “mic”, micronucleus; “Mac”, macronucleus; “om”, old macronucleus. Scale bars = 10μm apply to all panels in the set.