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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Vis Exp. 2016 Jan 2;(107):10.3791/53692. doi: 10.3791/53692

Figure 3. Expression of markers indicative of cell cycle arrest and differentiation in iMOP-derived sensory epithelia.

Figure 3

Otospheres of iMOP cells cultured in iMOP culture media. Nuclei of cells were labeled with (A) Hoechst and (B) Cdkn1b antibody. Filamentous actin was labeled with (C) phalloidin. (D) The merged image of a typical otospheres containing proliferating iMOP cells. Otospheres from iMOP cells were cultured in sensory epithelia culture media for 10 days. Cells from otopsheres were marked with (E) Hoechst, (F) Cdkn1b and (G) phalloidin. (H) Merged image of otospheres showing increased Cdkn1b and phalloidin labeling. Otospheres differentiated into sensory epithelia were also labeled (I) Hoechst, (J) Cdh1, (K) phalloidin. (L) The merged image shows typical strong Cdh1 labeling in the otospheres. Length of scale bars are 10 μm unless indicated.