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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Vis Exp. 2016 Jan 2;(107):10.3791/53692. doi: 10.3791/53692

Table 1. Reagents used for culture and differentiation of iMOP cells.

Name of the Reagent/Equipment Company Catalog Number Comments/Description (optional)
Tissue Culture Treated 24 Multiwell Plate Thermo Fisher Scientific 130188
Tissue Culture Treated 60 mm Dish Thermo Fisher Scientific 130181
Moxi Z Mini Automated Cell Counter Orflo MXZ001
Moxi Z Cassette Type S Orflo MXC002
DMEM/F12 Life Technologies 11320-033
Neurobasal Medium Life Technologies 10010-023
Phosphate Buffered Saline (PBS) pH 7.4 Life Technologies 14025-092
Hank's Balanced Salt Solution (HBSS) Life Technologies 17504-044 Stored as 1 ml aliquots
B27 Supplement (50X) Serum Free Life Technologies 25030-081 Stored as 5 ml aliquots
L-Glutamine(200 mM) Life Technologies 23017-015 Stored as 1 mg/ml 100 μl aliquots
Recombinant Murine Fibroblast Growth Factor, basic (bFGF) Peprotech 450-33 Resuspended per manufacturerer and stored as 20 mg/ml 20 μl aliquots
Carbenicillin, Disodium Salt Fisher BP2648-1
Natural Mouse Laminin Life Technologies P36934
Poly-D-Lysine Life Technologies 11320-082
CoolCell LX Alcohol-Free Cell Freezing Containers BioCision BCS-405
Cryogenic Vials (2 ml) Corning 430654
1.5 Thickness Glass Coverslip (Round 12 mm) Electron Microscopy Sciences 72230-01
Prolong Gold Antifade Mountant VWR 47743-736 Stored as 10 mg/ml 100 μl aliquots