Fig. 2.

Foxp3 and CD25 expression does not correlate to proliferation in peripheral blood CD8⁺ and CD4⁺ T lymphocytes. Peripheral blood mononuclear cells from 13 healthy (HIV-1-uninfected) subjects were assessed for proliferative status (Ki-67 expression) and CD25/Foxp3 expression in CD4⁺ and CD8⁺ T lymphocytes by flow cytometry. Ki-67 expression was observed in 1.82 ± 0.17% versus 1.67 ± 0.15% of CD4⁺ and CD8⁺ T lymphocytes respectively (not shown). (A, B) Representative plots are shown for CD4⁺ (A) and CD8⁺ (B) T lymphocytes. The upper plots show Foxp3 versus Ki-67 expression, and the lower subplots show Foxp3 and CD25 expression on gated Ki-67⁺ and Ki-67⁻ subpopulations (percentages indicate frequency within total CD4⁺ or CD8⁺ T lymphocytes). (C) Data from all 13 subjects are summarized.