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. Author manuscript; available in PMC: 2018 Jan 5.
Published in final edited form as: Org Biomol Chem. 2017 Aug 9;15(31):6640–6644. doi: 10.1039/c7ob01707c

Figure 4.

Figure 4

(A) The oxoTCO-tetrazine cycloaddition using a recombinant protein substrate containing a site-specifically incorporated tetrazine. Experiments were carried out both in vitro (PBS) and in vivo (E. coli) using a 2.2 : 1 eq/ax mixture of oxoTCO diastereomers. (B) Second order rate constants (k2) were determined under pseudo-first order conditions using ca. 100–260 equivalents of oxoTCO 3 by monitoring the increase in GFP fluorescence. (in vitro study shown here) (C) Quantitative determination of the cycloadduct was confirmed by ESI-MS.