| 10x TaqMan RT buffer | 5 μl |
| Random hexamers | 2.5 μl |
| MgCl2 (25 mM) | 11 μl |
| dNTPs | 10 μl |
| RNase Inhibitor | 1 μl |
| RTase | 1.25 μl |
| Nuclease-free H2O | 8.25 μl |
| Total volume | 39 μl |
| Use immediately after preparation | |
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