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. Author manuscript; available in PMC: 2017 Nov 29.
Published in final edited form as: Immunol Cell Biol. 2017 Sep 11;95(10):933–942. doi: 10.1038/icb.2017.74

Figure 2.

Figure 2

The Il7r−/− thymus has disorganized corticomedullary structure and a paucity of DCs. Thymuses were dissected from WT and Il7r−/− mice and snap frozen in OCT freezing media. 10 μm sections were cut and stained with anti-cytokeratin 8 (K8)/anti-rat conjugated to Cy3 (cTECs, red), anti-cytokeratin 5 (K5)/anti-rabbit conjugated to Cy5 (mTECs, blue), and anti-CD11c directly conjugated to FITC (DCs, green). Tiled images were gathered using an Axiovert microscope, shown at either 10X (A) or 20X (B).