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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: NPJ Regen Med. 2017 Jul 27;2:22. doi: 10.1038/s41536-017-0027-y

Figure 4. Macrophage depleted animals feature enhanced fibroblast differentiation and collagen maturation.

Figure 4

A. Alpha smooth muscle actin (αSMA) positive myofibroblasts populated the cryo-lesion in Clo-Lipo treated animals where αSMA+ cells were restricted to the epicardium in PBS-Lipo control lesioned animals at 7dpi. B. Discoidin Domain Receptor Tyrosine Kinase 2 (DDR2) expression was up-regulated in Clo-Lipo treated animals 7dpi at different sites at along the wound margin. C. Dissected 14dpi cryo-injured whole mounted hearts imaged under a polarizing lens at maximum contrast. Clo-Lipo hearts showed enhanced accumulation of cross-linked collagen (top panel) Picrosirius red stained sections visualized under polarized light showed accumulation of thick collagen bundles in Clo-Lipo treated animals at 14dpi. Scale bar = 100μM.