Figure 4. Thrombin generation and vascular permeability in Meox2Cre-Thbd-null mice.

(A) Calibrated automated thrombinography of plasma from control mice (Thbd^loxP/+, orange, n = 4) and Thbd-deficient mice (Thbd^−/−; blue, n = 4). (B) Thbd-null mice displayed prolonged lag time and time to peak thrombin generation (ttPeak), reduced endogenous thrombin potential (ETP), and peak thrombin generation (Peak). (C–D) Evans blue extravasation assay showing increased vascular permeability in the lungs of Thbd-null mice compared with controls Thbd^loxP/loxP; n = 5 per group). (D) Data shown in panel C were generated by quantitative scanning of fluorescence (at 720 nm) of whole-organ lung tissue.