Table 2.
Hemostatic status of Meox2Cre-Thbd-null mice with and without expression of the murine PC transgene, as compared with controls
| Control | Thbd-null | Thbd-null hmPC+ | hmPC+ | |
|---|---|---|---|---|
| D-dimer, median (IQR), ng/mL | 95 (86–139) (n = 15) | 135 (130–159) (P = .056) (n = 8) | 97 (81–145) (n.s.) (n = 8) | 95 (89–124) (n.s.) (n = 9) |
| TAT, median (IQR), μg/L | 27 (15–81) (n = 10) | 74 (41–119) (n.s.) (n = 4) | 14 (13–17) (n.s.) (n = 8) | 23 (10–52) (n.s.) (n = 9) |
| Fibrinogen, mean ± SD, % | 100 ± 16 (n = 5) | 51 ± 32 (p 0.011) (n = 6) | – | – |
| IL-6, median (IQR), pg/mL | 19 (14–27) (n = 14) | 162 (60–369) (P = .0011) (n = 8) | 244 (69–320) (P = .0016) (n = 12) | 17 (13–88) (n.s.) (n = 9) |
| Platelets, mean ± SD, × 109/L | 279 ± 81 (n = 12) | 223 ± 62 (n.s.) (n = 11) | – | – |
| Hemoglobin, mean ± SD, g/dL | 13.0 ± 0.7 (n = 12) | 11.7 ± 0.5 (n.s.) (n = 11) | – | – |
| White blood cells, mean ± SD, × 109/L | 6.3 ± 0.8 (n = 12) | 8.6 ± 1.3 (n.s.) (n = 11) | – | – |
| Activated platelets, median (IQR), % P-selectin+ αIIbβ3+ | 0.030 (0.009–0.25) (n = 5) | 0.065 (0.040–0.077) (n.s.) (n = 5) | – | – |
| Platelet leukocyte aggregates, median (IQR), % CD45/P-selectin + or CD45/αIIbβ3+ | 10.9 (9.4–11.0) (n = 5) | 16.0 (12.2–19.0) (n.s.) (n = 5) | – | – |
| Carotid injury thrombosis model | ||||
| Time to occlusion, median (IQR), s | 189 (163–203) (n = 8) | 538 (394–899) (P = 0.0413) (n = 3) | 226 (160–355) (n.s.) (n = 8) | 276 (238–392) (P < 0.001) (n = 7) |
Controls comprised Thbd wild-type and ThbdloxP/+ C57black mice. Normally distributed parameters are presented as means and standard deviations; non–normally distributed parameters are presented as medians and IQRs. hmPC+, mice expressing the PC transgene; IQR, interquartile range; n.s., nonsignificant; SD, standard deviation.