Fig. 2. Comparative ligandomics to identify Scg3 as a disease-selective ligand.

(A) Ligandomics globally maps cell-wide endothelial ligands with simultaneous binding activity quantification in live mice. (B) Quantitative comparison of the entire ligandome profiles for diabetic vs. healthy retina systematically identifies disease-associated endothelial ligands. (C) Comparative ligandomics identified Scg3 as a diabetes-high ligand, HRP-3 as a diabetes-low ligand and VEGF as a diabetes-unchanged ligand in 4-month-diabetic mice in term of their preferential endothelial binding activity. *** p<0.0001, χ² test, diabetic vs. healthy.