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. Author manuscript; available in PMC: 2018 Jan 28.
Published in final edited form as: J Vis Exp. 2017 Jul 28;(125):10.3791/56031. doi: 10.3791/56031

Figure 1. A comparison of the vertical and the horizontal native gels for analyzing the binding of Bicc1 to Cripto1 RNA.

Figure 1

Bicc1 binding experiments were conducted with a 32-nucleotide fluorescent CyclinB1 RNA negative control or a fluorescent 32-nucleotide Cripto1 RNA positive control. Each reaction contained either 0 nM or 250 nM of SUMO-Bicc1 N-terminal and analyzed on either a (A) vertical polyacrylamide native gel run for 30 min at 120 V at 4 °C or as duplicate samples in the horizontal polyacrylamide native gel for (B) 2 h at 120 V at 4 °C or (C) 5.5 h at 120 V at 4 °C. Please click here to view a larger version of this figure.