Table 1.
Primers and probes used in the multiplex TaqMan assay used to screen ear biopsy cultures for Borrelia spp. and to differentiate Peromyscus spp. for this study
| Primers and probes | Sequence 5′–3′a | Reference |
|---|---|---|
| Borrelia spp. | ||
| fliD-F | TGGTGACAGAGTGTATGATAATGGAA | Hojgaard et al. 2014 |
| fliD-R | ACTCCTCCGGAAGCCACAA | Hojgaard et al. 2014 |
| fliD-probe | FAM-TGCTAAAATGCTAGGAGATTGTCTGTCGCC-BHQ1 | Hojgaard et al. 2014 |
| 23S-F | TCGGTGAAATTGAAGTATC | This study |
| 23S-R | CARGCTATAGTAAAGGTTCA | This study |
| 23S-probe | HEX-CGTCTAACCACAAGTAATCGGCATC-BHQ1 | This study |
| purB-F | TCCTCAATGATGAAAGCTTTA | Graham et al. 2016 |
| purB-R | GGATCAACTGTCTCTTTAATAAAG | Graham et al. 2016 |
| purB-probe | CalRd610-TCGACTTGCAATGATGCAAAACCT-BHQ2 | Graham et al. 2016 |
| mIGS-F | TGTCGTTATCGGTATGTG | This study |
| mIGS-R | AAGGGCCATGATGATTTG | This study |
| mIGS–probe | Quas705-CCTTCCTACGACTTATCACCGACAG-BHQ3 | This study |
| Peromyscus spp. | ||
| Pero_F | ACAGAYGCCATCCCAGGCC | This study |
| Pero_R | AACGCTTTAAGCTTCACAGTG | This study |
a
HEX, hexachlorofluorescein phosphramidite; FAM, 6-carboxyfluorescein; CalRd610, CalFluor Red 610; Quas705, Quasar 705; BHQ1, BHQ2, and BHQ3 is Black Hole Quencher 1, 2, and 3, respectively.