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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Mol Plant Pathol. 2018 Jan 30;19(6):1427–1443. doi: 10.1111/mpp.12626

Fig. 6.

Fig. 6

Infection in Prunus persica by wild-type PPV (sf-WT) and PPV mutants carrying changes that prevent (sf-4SA) or mimic (sf-4SD) CP phosphorylation at serines 25, 81, 101 and 118. P. persica seedlings were inoculated by bombardment with viral cDNA. (A) Images of upper uninoculated leaves of plants infected with the indicated viruses, at 21 dpi, under an epifluorescence microscope. Bar, 5 mm. (B) PPV CP-specific immunoblot analysis of extracts from upper uninoculated leaves for individual or pooled plants, collected at 21 dpi. Viruses used as inocula and volume of extract loaded are indicated. Blots stained with Ponceau red showing the RuBisCO protein are included as loading control.