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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Mol Plant Pathol. 2018 Jan 30;19(6):1427–1443. doi: 10.1111/mpp.12626

Fig. 8.

Fig. 8

Infection of Nicotiana benthamiana and Prunus persica plants by wild-type PPV and PPV mutants bearing phosphorylation-mimicking mutations in the CP protein. Plants were inoculated by hand-rubbing (N. benthamiana) or bombardment (P. persica) with viral cDNA. (A, C) Images of upper uninoculated leaves of N. benthamiana (A) or P. persica (C) plants after infection with the indicated viruses, taken at 21 or 23 dpi, respectively, under an epifluorescence microscope. Bar, 5 mm. (B, D) PPV CP-specific immunoblot analysis of extracts from upper uninoculated leaves of N. benthamiana (B) or P. persica (D) individual or pooled plants, collected at 21 (N. benthamiana) or 23 dpi (P. persica). Inoculated viruses and extract volumes loaded are indicated. Blots stained with Ponceau red showing the RuBisCO protein are included as loading control.