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Prolonged stimulation with high glucose gives rise to multiple pulses of secretion islets, during which granules transiently appear. Pulses of secretory activity can be seen every ~ 3 – 4 min. Confocal plane of a human islet is shown. A pseudocolor scheme was applied for fluorescence intensity. Scale bar = 20 µm. Movie speed = 30 fps. Total duration of the movie is 20 min.
