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. Author manuscript; available in PMC: 2017 Nov 20.
Published in final edited form as: J Vis Exp. 2017 Sep 13;(127):10.3791/56089. doi: 10.3791/56089

Figure 5. High Glucose Triggers NPY-pHluorin Fusion with the Plasma Membrane.

Figure 5

(A) Maximal projection of confocal images of an intact human islet infected with NPY-pHluorin in basal glucose concentration (3G – 3 mM Glucose, left panel) or in high glucose (16G – 16 mM Glucose, right panel). Extracellular solutions contained the cAMP raising agents forskolin and IBMX (see Protocol for details). Scale bar = 10 µm. (B) Traces showing changes in mean fluorescence intensities (arbitrary units) in the green channel in ROIs placed around single secretory events (red, granule), cells (green) or cell clusters (blue). High glucose was applied for 5 min after ~ 2.5 min in 3G. Fluorescence values were normalized to the initial fluorescence value (baseline).