ConA-induced hepatic expression of cytokines and chemokines in HSC-depleted mice. At 6 hours after ConA administration, relative mRNA expression of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, IFN-β, CXCL1, and CXCL10 in HSC-depleted (HSC-depl) and HSC-sufficient (HSC-suff) mice was determined by quantitative real-time RT-PCR (A and C), and corresponding protein levels were measured by enzyme-linked immunosorbent assay (B and D). Data are expressed as means ± SD. n = 6 to 8 mice per group. *P < 0.05, **P < 0.01, and ***P < 0.001 versus control; †P < 0.05, ††P < 0.01, and †††P < 0.001 versus HSC-depleted mice.