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. Author manuscript; available in PMC: 2017 Dec 4.
Published in final edited form as: Mol Oncol. 2017 Nov 16;11(12):1806–1825. doi: 10.1002/1878-0261.12151

Fig. 6.

Fig. 6

Upregulated RNF44 and downregulated AMPK-α1 sensitize BR xenograft tumors to ADI-PEG20 treatment. (A) (B) Corresponding to our previous study (Li, et al., 2006), ADI-PEG20 significantly reduced BR tumor sizes by inducing cell apoptosis. Female nude mice were inoculated subcutaneously with 1 × 106 melanoma cells. When tumor volume reached 100 mm3, tumor bearing mice received treatment of ADI-PEG20 (100 IU/kg) twice per week. On day 32, single-cell suspensions were prepared from tumor tissues and then were labeled with Annexin V and then analyzed by FACS. (C) (D) AMPK-α1 and RNF44 expressions were detected by IHC staining and the data were presented as H-scores (scale bar = 100 μm) (n = 5, *p < 0.05, **p < 0.01, and ***p < 0.005).