Fig. 6.
Two different mechanisms of O-M gating at negative and positive voltages. (A) The two channels with Ser at position 77 at the cytosolic gate (KcvS, filled blue circles, and KcvNTS G77S, open black circles) have higher values for kOM (channel closing) at positive voltages than those channels with Gly at position 77 (KcvS S77G, open blue circles) and KcvNTS, filled black circles). The voltage dependence at negative voltages is not affected by the mutations, neither is the rate constant of channel opening, kMO (B). N = 3 for KcvS, KcvS S77G and KcvNTS G77S, n = 6 for KcvNTS wt.