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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Horm Cancer. 2017 Apr 10;8(3):135–142. doi: 10.1007/s12672-017-0294-5

Figure 6.

Figure 6

Co-targeting Ral-Fum hybrids block proliferation, clonogenic growth and mammosphere formation of breast cancer cells. (A) MCF-7 cells were treated with various drug concentrations in the presence of E2 (10nM) for 72hrs. Each drug’s inhibitory activity was calculated as % of E2 alone, which is set to 100%. (B) MCF-7 cells are seeded as single cells in estrogenized growth media (GM), then treated with the indicated concentration every 3–4 days for 2 weeks. Colonies were stained and quantified with ImageJ. Each drug’s inhibitory activity was calculated as % of GM alone, which is set to 100%. (C) MCF-7 cells were seeded as single cells on low attachment plates in MS media. DMSO (Veh) or drugs were added next day, and 7 days later the number of MS (MS>75μm in diameter) was quantified. ** P<0.01.