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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Genes Dis. 2017 Nov 2;4(4):229–239. doi: 10.1016/j.gendis.2017.10.004

Figure 3. Sox9 inhibits BMP2-induced osteogenic marker, but potentiates BMP2-induced chondrogenic marker.

Figure 3

(A & B). Western blotting analysis of OPN and Col2aI expression. C3H10T1/2 cells were infected with the indicated adenoviral vectors. At 7 days after infection, total cell lysate was prepared and subjected to Western blotting analysis with an OPN, Col2al, or β-actin antibodies (A). Western blotting results were quantitatively analyzed by using Quantity one (B). (C). qPCR analysis of OPN and Col2aI expression. C3H10T1/2 cells were infected with the indicated adenoviral vectors. Total RNA was isolated at 5 days after infection and subjected to quantitative RT-PCR (qPCR) using Col2al and OPN-specific primers. Gapdh was used as a reference gene. “#” p<0.05, vs. Ad-BMP2; “*” p<0.05, vs. Ad-GFP group.