Table 4.

Biological Pathways regulated by Target Genes (TGs) participating in significant miRNA-TF-TG interactions in normal colonic mucosa and differential colonic mucosa.

Normal TG	Differential TG	Biological Pathway1
	CCT5, MCFD2, PDIA6	cellular protein metabolic process
RAD51	MYC	cellular response to DNA damage stimulus
RAD51	SSRP1	DNA repair
RRM2	SSRP1	DNA replication
	GNB2, MYC	energy reserve metabolic process
RCC1, RRM2		G1/S transition of mitotic cell cycle
	CNOT6, MYC, SSRP1	gene expression
	GMPS, IMPDH2	GMP biosynthetic process
HELLS	IMPDH2	lymphocyte proliferation
HELLS, RCC1		mitosis
HELLS, IGF1R, VEGFA	MYC	negative regulation of apoptotic process
VEGFA	MYC	negative regulation of transcription from RNA polymerase II promoter
RRM2	GMPS, IMPDH2	nucleobase-containing small molecule metabolic process
RRM2	IMPDH2, LDHA	oxidation-reduction process
IGF1R, VEGFA		positive regulation of cell migration
IGF1R, VEGFA	CNOT6, MYC, NACC1	positive regulation of cell proliferation
VEGFA	MYC	positive regulation of epithelial cell proliferation
VEGFA	MYC	positive regulation of mesenchymal cell proliferation
IGF1R, VEGFA		positive regulation of protein kinase B signaling
VEGFA	MYC	positive regulation of transcription from RNA polymerase II promoter
	CCT5, PDIA6	protein folding
RAD51	NACC1	protein homooligomerization
CPM	ADAM28	proteolysis
	GMPS, IMPDH2	purine nucleobase metabolic process
	GMPS, IMPDH2	purine nucleotide biosynthetic process
	GMPS, IMPDH2	purine ribonucleoside monophosphate biosynthetic process
	CNOT6, MYC, SSRP1	regulation of transcription, DNA-dependent
RRM2	GMPS, GNB2, IMPDH2, LDHA	small molecule metabolic process
HELLS	CNOT6, MYC, NACC1	transcription, DNA-templated
RCC1	SSRP1	viral process

¹Pathways that were regulated by more than one TG were included in the table. Lines shaded in gray contain pathways that were identified for more than one TG in both normal and differential colonic mucosa.