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. Author manuscript; available in PMC: 2018 Jan 17.
Published in final edited form as: Int J Cancer. 2017 Oct 10;142(3):561–572. doi: 10.1002/ijc.31067

Figure 2. Characterization of M14 using Immunostaining and ABO Analysis.

Figure 2

A-B. Representative images of M14 cells in culture. Scale bars, 100 μm.

C-D. Immunostaining of M14 cells using a pan-melanoma antibody cocktail. Antibodies are directed against melanosome (HMB45), MART-1/Melan A (A103) and tyrosinase (T311). Cells displayed abundant cytoplasm and were multinucleated; some apoptotic cells were noted, as indicated by the arrows. Scale bar in C, 10 μm; scale bar in D, 20 μm.

E. ABO sequence demonstrating blood type O. Upper panel, sequence reported previously for A or B alleles and sequence reported for the O allele (21). Lower panel, forward and reverse sequence from M14 sample (passage 16, derived from passage 15 from 2 December 1975). Identical sequence results were obtained from ML14 and MDA-MB-435S samples (data not shown).