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. Author manuscript; available in PMC: 2009 Jul 7.
Published in final edited form as: J Neurosci. 2009 Jan 7;29(1):118–130. doi: 10.1523/JNEUROSCI.3985-08.2009

Figure 5.

Figure 5

Accumulation of polyubiquitinated proteins in the spinal cord of 12-month-old moca−/− mice. A, Western blotting patterns of ubiquitinated proteins from different brain regions of control (+/+) and moca−/− (−/−) mice. CTX, cerebral cortex; SC, spinal cord. Arrowhead indicates monomer ubiquitins. Polyubiquitin-conjugated high molecular weight proteins are indicated by the bracket. B, A representation of Triton X-100-soluble and -insoluble fractions of protein extracts followed by immunoblotting with ubiquitin antisera. C, The data are quantified from six independent experiments. GAPDH is the loading control. The percentage change in the expression of polyubiquitin-conjugated high molecular weight proteins (bracket) in the Triton X-100-insuluble fraction was normalized to GAPDH and shown as the mean ± SD. Statistical analysis was done by a Student’s t test (n = 6, *p < 0.05). D–G, Ubiquitin staining of the posterior funicular gray area of the spinal cord at the age of 2 months (D, E) and at the age of 20 months (F, G). Abnormal axonal spheroids stained by ubiquitin are present in moca −/− mice at the age of 20 months (G) but not at the age of 2 months (E). D, F, the age-matched controls. Scale bar, 50 μm. Ub, ubiquitin-containing aggregates.