Skip to main content
. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Cancer Immunol Immunother. 2018 Jan 13;67(4):639–652. doi: 10.1007/s00262-018-2115-2

Figure 3. Dual conditioning prolongs donor T cell proliferation and increases accumulation in the PLN.

Figure 3

a Percentage of CD90.1+ or TetI+ TCR-I cells accumulating in the PLN over time. N=6–18/group (Days 3–10), 2–3/group (Days 14, 21): WBI+αCD40+TCR-I vs. TCR-I (*), vs. WBI+TCR-I ($), vs. αCD40+TCR-I (&); 1 digit p<0.05, 2 digits p<0.01, 3 digits p<0.001, 4 digits p<0.0001 by two way ANOVA with Bonferroni corrections. b Representative flow cytometry panels from a. Numbers indicate the mean percentage +/− SEM of CD8+ cells. c CFSE staining of CD90.1+ TCR-I cells in the PLN 3 and 5 days post treatment. d Ki67 staining of CD90.1+ TCR-I cells in the PLN 7 days post treatment. N=4–6/group; WBI+αCD40+TCR-I vs. all other groups, *p<0.05 by one way ANOVA with Dunnett’s post-test.