Table 2. The effects of viral vector expression, hormonal treatment, and exposure to K+ on striatum dialysate concentrations of GABA.
Electropherograms were collected and the mean was obtained before (basal) and after (post-injection) an injection of 5 9μg EB or vehicle to OVX female rats. There was no main effect of treatment (F1,539 = 4.34, p = 0.061) on basal GABA concentration, and no interaction between treatment and time of sample collection (F49,539 = 0.68, p = 0.949). Following recovery from the K+ stimulation the GABA dialysate concentration was also determined. Basal extracellular GABA concentrations in dialysate were compared with pre-injection and pre-stimulation basal GABA concentrations in same rats. Two way ANOVA with repeated measures showed that that there was no main effect of treatment (F1,550 = 4.13, p = 0.067) on post-stimulation of basal GABA concentration and no interaction between treatment and time of sample collection (F50,550 = 0.75, p = 0.894).
| Group | Basal GABA in μM ± SEM | GABA Post-EB Injection in μM ± SEM | GABA Post recovery from K+ Stimulation in μM ± SEM |
|---|---|---|---|
| AAV.ALP + OIL | 0.11 ± 0.01 | 0.109 ± 0.008 | 0.088 ± 0.009 |
| AAV.ALP + EB | 0.14 ± 0.03 | 0.12 ± 0.02 | 0.11± 0.02 |
| AAV.ERα + OIL | 0.10 ± 0.01 | 0.09 ± 0.01 | 0.08± 0.01 |
| AAV.ERα + EB | 0.10 ± 0.02 | 0.07 ± 0.01 | 0.07± 0.02 |