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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Oncogene. 2018 May 2;37(32):4385–4397. doi: 10.1038/s41388-018-0278-0

Figure 2. TRC105 induces transient DNA damage and repression of SIRT1 induction.

Figure 2

22Rv1 were pre-treated with IgG or TRC105 24 hours prior to irradiation with 4 Gy. a. γ-H2AX or 53BP1 were immunolocalized at 4, and 24hours post-irradiation. Foci per nuclei were quantified (n = 100). Representative images are shown for γ-H2AX (green), and 53BP1 (green) foci, nuclear counterstained with DAPI (blue). b. Comet assay was performed 30 minutes and 24 hours following irradiation. The tail moment was quantified (n = 50). c. The mRNA expression of DNA damage repair genes were measured by rtPCR 72 hours post-irradiation. d. SIRT1 mRNA expression was measured in a time course 0–72 hours following 4 Gy irradiation of 22Rv1. SIRT1 mRNA expression was normalized to GAPDH and to untreated. Data are reported as a mean +/− S.D. of 3 independent experiments (***p < 0.001, ****p < 0.0001, NS – not significant, compared to control).