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. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: NPJ Precis Oncol. 2017 Jul 25;1:24. doi: 10.1038/s41698-017-0028-8

Fig. 5.

Fig. 5

KRAS mutation detection. a Schematic of the polymerase chain reaction/ligase detection reaction (PCR/LDR) assay. b Electropherograms of LDR products for: No gDNA; HT29 wt35 (50 nt); LS180 G35A (44 nt); M-PDAC CTCFAPα G35A (44 nt), CTCEpCAM G34C (61 nt); and CTCFAPα G35T (55 nt). The gray trace shows the DNA markers. The fluorescence intensity values are arbitrary. c Table summarizing PCR/LDR results for HT29 and LS180 cell lines, M-CRC, L-CRC, M-PDAC, and L-PDAC CTCs. d RT-qPCR gene expression profiles for L-PDAC patient #66 and M-PDAC patient #67