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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Eur J Immunol. 2017 Oct 20;48(2):306–315. doi: 10.1002/eji.201747092

Figure 2. PTPN22 regulates T cell dependent IL-17 responses induced by curdlan stimulated BMDC in vivo.

Figure 2

Wild type (WT) and Ptpn22−/− derived bone marrow derived dendritic cells (BMDC) were pulsed overnight with OVA323-339 (50nM) in the presence or absence of curdlan (100μg/ml). BMDC were harvested and injected into the left footpad of OT-II mice. 7 days post immunisation draining popliteal lymph nodes were isolated and the number of cells within the draining (A) lymph nodes determined. Total draining (B) lymph node T-cells were stimulated with immobilised anti-CD3 for 48 hours and cell-free supernatant assayed for IL-17, IFNγ and TNFα by immunoassay. Data are representative of three independent experiments, each data point representing an individual OT-II mouse lymph node. Bars represent the mean ± s.d. NS = not significant, *p<0.05, **p<0.01, ***p<0.001 by two-way ANOVA, applying Sidak’s multiple comparisons test.