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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Eur J Immunol. 2017 Oct 20;48(2):306–315. doi: 10.1002/eji.201747092

Figure 3. Ptpn22 regulates BMDC IL-1β secretion in response to curdlan.

Figure 3

WT and Ptpn22−/− derived bone marrow derived dendritic cells (BMDC) were stimulated for 24 hours in the presence or absence of curdlan (100 μg/ml). Cell-free supernatants were assessed for IL-1β, IL-6, IL-12/23p40, and TNFα by immunoassay. Data are pooled from 8-12 independent experiments, and represent mean ± s.d; NS = not significant, **p<0.01, ***p<0.001 applying two-way ANOVA, with Sidak’s multiple comparisons test. (B) WT BMDC were incubated in the presence or absence of α-dectin-1 (20 μg/ml) or IgG2a isotype control antibody for 30 minutes prior to stimulation for 24 hours in the presence or absence of curdlan (100 μg/ml). Cell-free supernatants were assessed for IL-1β by immunoassay. Data are pooled from 5 independent experiments, and represent mean + s.e.m.; NS = not significant, *p<0.05, **p<0.01 applying one-way ANOVA, with Holm-Sidak’s multiple comparisons test.