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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Eur J Immunol. 2017 Oct 20;48(2):306–315. doi: 10.1002/eji.201747092

Figure 5. Ptpn22 regulates dectin-1 dependent Syk and Erk activation in BMDC.

Figure 5

(A-C) WT and Ptpn22−/− BMDC (6×106 c/ml) were stimulated with heat killed C. albicans (HKCA) (6.25 ×105 c/ml) at 37°C for the indicated time points. Whole cell lysates were resolved by SDS-PAGE and immunoblotted using specific antibodies to pSyk (Tyr525/526) or Syk, and pErk1/2 (p42/44) or Erk. Quantification of HKCA induced band intensity measurements pooled from 5 independent experiments are shown for pSyk (B) and pErk (C), relative to Syk and Erk, respectively. Phosphorylated Syk protein values were normalised to total protein and the fold change to 0 min calculated. Data are means + s.e.m pooled from 5 independent experiments. * p<0.05 applying two-way ANOVA, with Sidak’s multiple comparisons test.