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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Eur J Immunol. 2017 Oct 20;48(2):306–315. doi: 10.1002/eji.201747092

Figure 6.

Figure 6

Ptpn22R619W regulates T cell dependent IL-17 responses induced by curdlan stimulated BMDC in vivo. WT and Ptpn22R619W derived bone marrow derived dendritic cells (BMDC) were pulsed overnight with OVA323-339 (50nM) in the presence or absence of curdlan (100μg/ml). BMDC were harvested and injected into the left footpad of OT-II mice. 7 days post immunisation the non-draining and draining popliteal lymph nodes were isolated. Total draining draining lymph node T-cells were stimulated with immobilised anti-CD3 for 48 hours and cell-free supernatant assayed for (A) IL-17, IFNγ and TNFα by immunoassay. Data are of two independent experiments, each data point representing an individual OT-II mouse lymph node. Bars represent the mean ± s.d.; NS = not significant, * p<0.05 by two-way ANOVA, applying Sidak’s multiple comparisons test. (B) WT and Ptpn22R619W GM-CSF derived BMDC were stimulated for 24 hours in the presence or absence of curdlan (100μg/ml). Supernatants were assessed for secretion of IL-1β and IL-6 by immunoassay. Data shown are mean ± s.d. Data are of 4 independent experiments; NS = not significant, *p<0.05, ***p<0.001 by two-way ANOVA, applying Sidak’s multiple comparisons test.