| Cycle | Temperature (°C) | Time |
|---|---|---|
| 1 | 72 | 5 min |
| 2 | 98 | 30 sec |
| 3 | 98 | 10 sec |
| 63 | 30 sec | |
| 72 | 1 min | |
| Go to step 3 | 10x | |
| 14 | 4 | hold |
Note: After the stopping point, samples can be
stored at −20 °C indefinitely.
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