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Golden Gate reaction for cloning gRNA cassette plasmid
| Reagent | Amount |
|---|---|
| gRNA on pYTK050 | 20 fmol |
| ConL1 (pYTK003) | 20 fmol |
| ConRE (pYTK072) | 20 fmol |
| AmpR-ColE1 (pYTK083) | 20 fmol |
| NEB T4 buffer 10× | 1.0 μl |
| NEB T7 ligase | 0.5 μl |
| NEB BsaI | 0.5 μl |
| ddH2O | to 10 μl |
