Table 1. Calculating percent repair using CT values generated from SSPE-qPCR.

(A) Repair of a DPC-containing substrate transfected in V79 Chinese hamster lung fibroblasts 3 and 8 h post-transfection. (B) SSPE-qPCR of 0 h samples not transfected into cells. In one sample the efficiency of the crosslinking reaction between DNA and oxoguanine glycosylase was high (this sample yielded a low delta CT value) while in the other sample the efficiency of protein crosslinking was low (this sample yielded a relatively high delta CT value). See text of Representative Results for details.

A
Time	− Primer extension	+ Primer extension	Δ CT	Percent Repair (2ΔCT/23× 100)
3 h	23.5	21.1	2.4	66
8 h	29.4	26.5	2.9	93

B
Time	− Primer extension	+ Primer extension	Δ CT	Percent Background (2ΔCT/23× 100)
0 h	15.4	14.6	0.8	22
0	15.4	12.9	2.5	71