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. Author manuscript; available in PMC: 2019 May 23.
Published in final edited form as: J Innate Immun. 2018 May 23;10(3):228–238. doi: 10.1159/000488914

Figure 2. Epithelial HO-1 regulates leukocyte-epithelial crosstalk.

Figure 2

(A) Model to examine epithelial-leukocyte interactions using polarized Caco-2 IECs in the upper chamber of a Transwell insert with THP-1 monocytes in the lower chamber, activated with S. Typhimurium for 6h for mRNA or 24h for protein. (B–D) Expression of IL-8, CCL20 and CXCL1 mRNA from Caco-2 IECs during co-culture with activated THP-1 cells. (E) IL-1β secretion from THP-1 cell supernatants after 24h, as measured by ELISA. (F) IL-1 β secretion from THP-1 cells co-cultured with Control shRNA and HMOX1 shRNA transduced Caco-2 IECs after 24h. Data examine physiologic bacterial-epithelial activation using polarized IECs in the upper chamber of a Transwell insert exposed to enteric bacteria in the presence (or absence) or leukocytes in the lower chamber, evaluating IEC chemokine expression. (H) Expression of IL-8 mRNA from Caco-2 IECs 6h after exposure of apical surface to pathogenic bacteria in presence or absence of THP-1 monocytes. Combined results from 3 independent experiments *, p <0.05; ***, p <0.001; ****, p <0.0001.