Skip to main content
. Author manuscript; available in PMC: 2018 May 14.
Published in final edited form as: Bio Protoc. 2018 Feb 5;8(3):e2721. doi: 10.21769/BioProtoc.2721

Figure 1. Overview of the workflow for precision tagging by combinational use of type II and type IIS restriction endonucleases.

Figure 1

A–E. GOI in different stages is specified in the precision tagging workflow. Type IIS DNA cassette in the GOI is highlighted in the red box. Type IIS restriction endonucleases are used between (C) and (D) and emphasized in blue frame. GOI is the abbreviation of ‘Gene of Interest’.