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. Author manuscript; available in PMC: 2018 May 14.
Published in final edited form as: Bio Protoc. 2018 Feb 5;8(3):e2721. doi: 10.21769/BioProtoc.2721

Figure 6. Schematic of making a tag clone from a template clone of THSD1.

Figure 6

A–B. The template clone contains two THSD1-specific sticky ends (letters in red or green). C. All above tags containing a THSD1-specific sticky end and 5′-phosphorylation are joined with the linearized template clone by T4 DNA ligase, thus generating a seamless tag clone.