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. Author manuscript; available in PMC: 2019 Jan 3.
Published in final edited form as: J Vis Exp. 2018 Jan 3;(131):10.3791/56711. doi: 10.3791/56711

Figure 1. Schematic of 3D tracking system.

Figure 1

The 2D-EOD (EOD1 & EOD2) and the TAG lens (TAG) deflect the laser along the XY and Z directions, respectively. The APD (APD) is used to collect fluorescence photons, which are sent to the FPGA (FPGA). FPGA is used for the position calculation algorithm, photon counting, control of the 2D-EOD as well as control and readout of piezoelectric nanopositioner (NSxy and NZz). Other components labeled in the figure: mirrors (M); lenses (L#); pinhole (PH); Glan-Thompson polarizer (GP); half-wave plate (W1); dichroic filter (DC); objective lens (OL, 100X NA = 1.49); fluorescence emission filter (F); beam splitter (BS); XY micropositioner stage (MSxy); Z micropositioner stage (MSz).