Skip to main content
. Author manuscript; available in PMC: 2019 Jan 3.
Published in final edited form as: J Vis Exp. 2018 Jan 3;(131):10.3791/56711. doi: 10.3791/56711

Figure 6. Precision of fixed particle tracking.

Figure 6

(a) A fixed particle is tracked by 3D-DyPLoT at different count rates. The precision is (b) 17.6 nm in X, (c) 26.4 nm in Y, and (d) 53.4 nm in Z at an emission rate of 100 kHz. (e) When probing slower processes, the feedback control constant (KI) can be reduced by a factor of 10 to increase precision. Under this reduced feedback control, the precision is (f) 6.5 nm in X, (g) 8.3 nm in Y, and (h) 10.5 nm in Z at an emission rate of 100 kHz.