Table 1.

Data processing and refinement statistics.

Data processing	mHRF	hHRF-1	hHRF-2
Space group	P 21 21 2	P 1 21 1	P 21 21 21
Unit cell dimensions (Å)	a = 69.53	a = 49.73	a = 47.53
	b = 99.85	b = 58.95	b = 77.59
	c = 57.47	c = 57.73	c = 99.32
		β= 99.41°
Resolution (Å)
Overall (outer shell)	57.06–4.01 (4.63–4.01)	24.53–1.75 (1.78–1.75)	19.86–1.40 (1.43–1.40)
Completeness (%)a	98.5 (95.8)	99.3 (97.3)	99.7 (99.6)
Multiplicitya	11.6 (12.0)	4.5 (3.4)	7.4 (7.6)
Mean ((I)/σ(I))a	9.0 (2.1)	11.7 (1.8)	12.7 (1.9)
Rmergea	0.271 (1.892)	0.124 (1.085)	0.066 (1.112)
Rpima	0.081 (0.554)	0.065 (0.667)	0.025 (0.428)
CC1/2a	0.997 (0.877)	0.988 (0.342)	0.999 (0.703)
Wilson B-factor (Å2)	233	15.9	15.1
Refinement
Rwork/Rfree (%)b	29.96/34.16	18.84/22.09	17.93/19.85
No. of reflections	3 125c	32 846	72 706
RMSD
Bond lengths (Å)	0.001	0.004	0.015
Bond angles (°)	0.361	0.632	1.546
Coordinate error (Å)	0.48	0.20	0.14
No. of atoms
Protein	2 159	2 564	2 581
Solvent	0	215	301
Other	6d	0	5e
Ramachandran plot
Favoured (%)	97.12	99.05	98.48
Allowed (%)	100	100	100

^aValues in parentheses are for the highest resolution shell.

^bR_free set comprises 5% of reflections.

^cData were truncated to resolution limits of 4.2 Å, 4.5 Å and 4.0 Å.

^dGlycerol.

^ePolyethylene glycol.