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. Author manuscript; available in PMC: 2018 May 31.
Published in final edited form as: FEBS J. 2017 Sep 11;284(19):3278–3301. doi: 10.1111/febs.14192

Fig. 3.

Fig. 3

Assessment of sdAbs specificity against heme versus heme analogs or heme contained in hemoproteins. Inhibitory effect of preincubation of sdAb with increasing concentrations of (A) FePP, ZnPP, CoPP or SnPP, (B) FePP or Fe2 (SO4)3 (C) FePP, Hb, MetHb, Mb, Cytochrome c (Cytc) or human serum albumin bound to heme (HSAH), measured by ELISA. Data from one out of three independent experiments with similar trend are shown.