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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: J Vis Exp. 2018 Apr 26;(134):10.3791/57498. doi: 10.3791/57498

Figure 1. Examination of amyloid-like fibers during necroptosis.

Figure 1.

Whole cell lysates were harvested from cells undergoing TNF-induced necroptosis and subjected to SDD-AGE. Western blots were performed for RIPK1, RIPK3, and MLKL. The MLKL-containing fibers exhibited a distinct migration pattern from the RIPK1/RIPK3-containing fibers. The MLKL monomer is barely visible under this condition.